093050044  MRA支原体去除试剂Mycoplasma Removal Agent 5ml

应用：预防支原体污染，支原体去除！

货名：支原体去处试剂 MRA

货号：093050044

规格：5ml

品牌：MP品牌

描述：细胞培养用品，原装

    在不损伤细胞的前提下消除支原体污染

●具有很强的抑制支原体活性的能力，从污染的培养中彻底清除支原体：

 MRA属于抗生素喹啉家族的衍生物，通过抑制DNA促旋酶清除支原体污染，该酶是微生物DNA复制过程中必不可少的酶类。

●活性范围广：

 MRA的活性在细胞培养物中可以维持长达7天，对多种支原体株系均有作用，并且浓度低至0.5ug/ml即可发挥功效。

●作用浓度低，细胞毒性小：

 在推荐浓度下使用MRA，细胞毒性极小，可以作为预防支原体污染使用的试剂。

●防止支原体污染复发：

 一旦使用了MRA，培养细胞就不会再受到同样的支原体污染，从而起到预防污染的作用。

●使用方便：

 MRA使用非常方便，只要添加到支原体污染的细胞培养物中即可。

MRA提供的浓度时即拆即用型，室温保存，每瓶MRA含有5ml4-oxo-quinoline-3-carboxylic acid衍生物。

Mycoplasma Removal Agent (MRA)
Unit Size: 5 ml (50 ug/ml)
1. Introduction
Mycoplasma Removal Agent (MRA) has been developed by Dainippon Pharmaceutical Co., Ltd. in Japan for cell culture. This agent has been shown to be
effective in the elimination of various types of mycoplasma from contaminated cultures. MRA can be used to prevent recontamination of cured cultures by
mycoplasma.
The product contains 50 ug of 4-oxo-quinoline-3-carboxylic acid derivative in 1 ml of water.
2. Features
1. MRA shows a strong anti-mycoplasma activity against various types of mycoplasma.
2. If cells are treated with MRA, recontamination of that culture with the original mycoplasma is not detected while preventative doses of MRA are in use.
3. MRA can be used very conveniently. Simply add to cell cultures contaminated by mycoplasma and incubate for a week.
4. As a preventative measure, MRA can be used to avoid mycoplasma contamination. MRA should not be used as a substitute for good cell culture
techniques.
3. Procedure
1. Add MRA to cell cultures contaminated by mycoplasma at a concentration of 0.5 ug/ml and incubate for a week. (Add 0.1 ml of MRA in the case of 10
ml of media in a 25 cm2 flask.)
2. Use a medium containing MRA at the same concentration for media replacement or culture transfer (passage).
3. Transfer the cell cultures several times without MRA and confirm that regrowth of the contaminating mycoplasma has not occurred.
4. A Mycoplasma detection kit (such as MP's Mycoplasma Hoechst Stain Kit, catalog number 3030000, or Immu-Mark Myco-Test Kit, catalog number
3020000) is recommended for the detection of contamination.
5. If there is a concern about the presence of mycoplasma in serum or trypsin, contamination of the cell cultures exposed to these products can be
prevented by adding MRA at a concentration of 0.1 ug/ml to the media.
4. Storage
MRA is stable at room temperature. Protect from light to prevent decomposition.
5. Caution Upon Use
1. MRA is a research reagent and must be used only as a mycoplasma removal agent in cell cultures. MRA is a synthetic molecule. Good laboratory
practices should be observed when handling the product.
2. The recommended concentration for use is 0.5 ug/ml. Concentration may be raised up to 1 ug/ml only when the recommended concentration is
ineffective in removing the mycoplasma. For preventative purposes, MRA can be used at 0.1 ug/ml.